刘灶长，上海市农业生物基因中心研究员，华中农业大学联培研究生导师，上海市浦江人才，上海市闵行区领军人才。

水稻抗旱分子生物学和资源生物科学

研究领域

        水稻逆境生物学和资源生物科学。

研究内容

1. 水稻节水抗旱的生理生态特征及其分子生物学基础；

2. 农业生物资源的收集、评价、鉴定和超低温保存技术；

3. 节水抗旱稻的栽培技术工艺与环境效应评价以及海外示范推广。

学术活动

       1. 中国作物学会水稻专业委员会委员

       2. 上海市生物工程学会会员

       3.  日本作物学会会员

拥有国家发明专利4项和实用新型专利1项。主持的种质资源超低温保存方面的研究成果曾获上海市技术发明二等奖。

在水稻特别是旱稻和节水抗旱稻中，通过干旱胁迫压力选择，开展了抗旱相关转录组学及相关基因的克隆与机能解析，鉴定和克隆了水稻干旱胁迫响应或节水抗旱的功能基因和调控基因如非编码miRNA，阐明相关基因的功能、相互作用及其在水稻节水抗旱中的应用潜力。助力基因中心植物种质资源特别是离体种质资源的超低温保存技术平台的建设。探索利用滴灌栽培节水抗旱稻的技术工艺、可行性与环境效应。抗旱新基因OsAHL和小RNA miR171、miR408等相关研究工作分别在Sci. Reports， JXB和遗传杂志发表。

1. Junguo Bi, Danping Hou, Xianxian Zhang, Jinsong Tan, Qingyu Bi, Kangkang Zhang, Yi Liu, Feiming Wang, Anning Zhang, Liang Chen, Guolan Liu, Zaochang Liu, Lijun Luo. 2021. A novel water-saving and drought-resistance rice variety promotes phosphorus absorption through root secreting organic acid compounds to stabilize yield under water-saving condition. Journal of Cleaner Production, Doi:10.1016/j.jclepro.2021.127992

2. Yu Zhang, Jia Li, Shoujun Chen, Xiaosong Ma, Haibin Wei, Chen Chen, Ningning Gao, Yuqiao Zou, Deyan Kong, Tianfei Li, Zaochang Liu, Shunwu Yu, Lijun Luo. 2020. Knockout of OsEBP89 enhances anaerobic germination and and drought stress tolerance via H2O2 level in rice. Molecular Genetics and Genomics, Doi:org/10.10077s00438-020-01669-7   

3. Jun Zhao, Cong Lu, Muhammad Tariq, Qinran Xiao, Wei Zhang, Kai Huang, Qiang Lu, Kuangfei Lin, Zaochang Liu. 2019. The response and tolerance mechanisms of lettuce (Lactuca sativa L.) exposed to nickel in a spiked soil system. Chemosphere, 222: 399-406.

4. Mingxia Fan, Chunyu Zhang, Lei Shi, Chen Liu, Wenjuan Ma, Meiming Chen, Kuichen Liu, Fengchun Cai, Guohong Wang, Zhengyi Wei, Min Jiang, Zaochang Liu, Ansar Javeed and Feng Lin. 2018. ZmSTK1 and ZmSTK2, encoding receptor-like cytoplasmic kinase, are involved in maize pollen development with additive effect.  Plant Biotechnology Journal, 16: 1402-1414. Doi:10.1111/pbi.12880.

5. Liguo Zhou^1,2*, Zaochang Liu^1,*,Yunhua Liu^1,*, Deyan Kong¹, Tianfei Li¹, Shunwu Yu¹, HanweiMei¹, Xiaoyan Xu¹，Hongyan Liu¹, Liang Chen¹, and Lijun Luo¹. 2016. A novel gene OsAHL1 proved to improve both drought avoidance and drought tolerance in rice. Scientific Report, 6, 30264; doi:10.1038/srep30264.

6. Deyan Kong, ShoujunChe, Liguo Zhou, HuanGao, Lijun Luo, Zaochang Liu. 2016. Research progress of photoperiod regulated genes on flowering time in rice. Hereditas, 38(6):532-542 (In Chinese with English abstract)

7. Modinat A. Adekoya, Zaochang Liu, Eli Vered, Liguo Zhou, Deyan Kong, Jianying Qin, Ruifang Ma, Xinqiao Yu, Guolan Liu, Lin Chen & Lijun Luo. 2014. Agronomic and ecological evaluation on growing water-saving and drought-resistant rice (Oryza sativa L.) through drip irrigation. Journal of Agricultural Science. 6 (5):110-119.

8. Lijuan Liu, Zaochang Liu (Corresponding author), Hairong Chen, Liguo Zhou, Yunhua Liu & Lijun Luo. 2012. SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties. Journal of Agricultural Science. 4 (3): 277-236. http://dx.doi:10.5539/jas.v4n3p227.

9. 秦剑英，刘灶长，孔德艳，周立国，罗利军. 2012. 适合于miRNA的荧光定量PCR优化体系的建立及其标准. 植物遗传资源学报, 13(3):435-442.

10. M. Fan^§, Z. Liu^§,*, L. Zhou, T. Lin, Y. Liu and L. Luo*. 2011. Effects of plant growth regulators and saccharide on in vitro plant and tuberous root regeneration of cassava (Manihot esculenta Crantz). J Plant Growth Regul. 30(1): 11-19.  DOI 10.1007/s00344-010-9163-y.

11. L. Zhou^§, Y. Liu^§, Z. Liu^§,*, D. Kong, M. Duan and J. Luo*. 2010. Genome-wide identification and analysis of drought-responsive microRNAs in Oryza sativa. J. Exp. Bot. 61 (15): 4157-4168.

12. H.R. Zheng¹, Z.C. Liu², et al. 2009. Induction of mutation in Jujube (Zizyphus jujuba Mill) using tissue culture combined with ⁶⁰Co-γRay irradiation. In: IAEA ed. Induced mutation in Tropocal Fruit Trees. IAEA-TECDOC. International Atomic Energy Agency, Vienna, ISSN 1011-4289. pp113-122.

13. 刘丽娟, 刘灶长, 陈海荣, 罗利军. 2009. SRAP标记技术及其在蔬菜作物遗传多样性分析中的应用, 中国农学通报, 25(21):43-48.  

14. 刘艳霞, 刘灶长, 林田, 李天菲, 陈发棣, Lee Inho, 罗利军. 2009. 菊花茎尖的玻璃化超低温保存研究. 植物遗传资源学报, 10(2):249-254.

15. 杨立勇, 刘灶长, 周立国, 罗华程, 罗利军. 2009. 高效、新T-DNA侧翼序列分离技术——Actail-PCR. 中国农业科学, 42(4):1447-1451.

16. 刘丽娟, 刘灶长, 陈海荣, 罗利军, 2008/6/16, 莴苣属蔬菜资源SRAP标记PCR体系的构建与优化, 植物遗传资源学报, (02)

17. 刘运华^1,2，刘灶长^1,*，罗利军^1,*．2007.植物miRNA 及其在植物发育进程和环境胁迫响应中的潜在功能. 植物生理学通讯，43(5): 987-992.

18. 刘运华^1,2，刘灶长^1,*，周立国^1,2，余舜武¹，刘鸿艳¹,罗利军^1,*．2007. 干旱胁迫下耐旱稻中旱3号全长cDNA文库的构建. Molecular Plant Breeding. 5 (3): 367-370.

19. 刘三雄^1,2，刘灶长^1,*，周立国^1,2，余舜武¹，刘鸿艳¹,朱天生^1,2,罗利军^1,*．2007. OsI2 基因的克隆及其植物表达载体的构建. Molecular Plant Breeding. 5 (4): 548-552.

20. 王艳芳,房伟民,陈发棣,赵宏波,刘灶长. 2007.‘神马’菊花的离体保存及遗传稳定性.西北植物学报，27(7):1341-1348.

21. 林田¹，刘灶长^1,*，李田菲¹，李锡香²，罗利军^1,*．2006． 红花石蒜茎尖的玻璃化超低温保存．植物生理学通讯，42(6): 1063-1066.

22. 林田¹，刘灶长^1,*，李田菲¹，郑海柔¹，罗利军¹．2006．不同激素配比对红花石蒜小鳞茎及茎尖的分化培养的影响, 上海农业科学,22(4):45-47.

23. 杨华^1,2，刘灶长^1,*，陈海荣¹，陈亮¹，罗利军^1,*．2006．上海地区芸薹属蔬菜遗传多样性研究. 植物遗传资源学报, 7(3):264-269.

24. B.J Park¹, Z.C. Liu ^{2, *}, A. Kanno¹ and T. Kameya¹.2005. Increased tolerance to salt and drought stress in transgenic lettuce (Lactuca sativa L) by constitutive expression of LEA gene. Plant Growth Regulation. 45:165-171.   

25. B. J. Park¹ · Z. C. Liu^2,* and T. Kameya¹. 2005. Transformation of radish (Raphanus sativus L.) via sonication and vacuum-infiltration of germinated seeds with Agrobacterium harboring a group 3 LEA gene from B. napus. Plant Cell Reports. 24: 494–500.Published online: 21 April 2005. 10.1007/s00299-005-0973-5                     

26. Byong-Jin Park^{a, 1}, Zaochang Liu^{b, 1,                                               ,} , Akira Kanno^a and Toshiaki Kameya^a. 2005. Genetic improvement of Chinese cabbage for salt and drought tolerance by constitutive expression of a B. napus LEA gene. Plant Science.  10.1016/j.plantsci.2005.05.008                    169(3):553-558. Published online: May 31, 2005.

Zaochang Liu^{2, *}, Byong-Jin Park¹, Akira Kanno¹, Toshiaki Kameya¹. 2005. The novel use of a combination of sonication and vacuum infiltration in Agrobacterium-mediated transformation of kidney bean (Phaseolus vulgaris L.) with lea gene. Molecular Breeding. 16 (3): 189- 197. DOI: 10.1007/s11032-005-6616-2

27. Z. C. Liu, H. H. Tong, H.Y. Liu, L. Chen, L.J Luo^*. 2004. The primary studies on gene expression of drought tolerance or sensitive rice cultivars in different water conditions. In: Poland D, Sawkins M, Ribaut J.-M, and Hoisington D (eds.) Resilient Crops for water limited environments: Proceedings of a workshop held at Cuernavaca, Mexico, 24-28 May, Mexico D.F: CIMMYT, pp 51-52.

28. Liu, Z., Goto, Y., Nishiyama, I and Kokubun, M. 2001. Effects of foliar- and root-applied benzylaminopurine on tillering of rice plants grown in hydroponics. Journal of Plant Production Science . Vol. 4(2):250-256.

29. Liu, Z., Goto, Y. and Nishiyama, I. 2000. Effect of benzylaminopurine on shoot and root development and growth of rice grown with different nitrogen forms.  Journal of Plant Production Science. Vol. 3(4):349-353.

30. Liu, Z., Goto, Y. and Nishiyama, I. 1999. Effects of benzylaminopurine added to the soil and fertilizer levels on growth of two rice cultivars: Sasanishiki and North Rose. In: Proceedings on International Symposium “World Food Security and Crop Production Technologies for Tomorrow”. Ed. T. Horie, S. Geng, T. Amano, etc. Graduate School of Agricultural Science, Kyoto University. 281-282.

授权专利

1. 刘灶长，林田，李天菲，郑海柔，罗利军. 一种营养繁殖花卉石蒜的超低温保存方法. 中国，发明专利，ZL200610113832.X. 2008年.

2. 刘灶长，刘艳霞，林田，李天菲，罗利军.菊花超低温保存方法. 中国，发明专利，ZL200810115360.0. 2011年.

3. 周立国，刘灶长，刘运华，孔德艳，秦剑英，罗利军. 水稻OsAHL蛋白及其应用. 中国，发明专利，ZL201010190824.1. 2012年.

4. 刘灶长，周立国，孔德艳，刘运华，罗利军. 一种可精确测定作物水分利用效率的栽培装置. 中国，实用新型，ZL201620100811.3. 2016年.

5. 周立国，刘灶长，孔德艳，罗利军. 水稻OsAHL基因启动子及包含其的重组载体、转化体及其应用. 中国，发明专利，ZL201510271470.6. 2018年.